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During this time, in vitro, in vivo, and in silico studies on this tetrapeptide have been performed, supporting the geroprotective and neuroendocrine nature of this peptide as result of its antioxidant, neuro-protective, and antimutagenic effects [13]

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In the current study, depriving RPE cells of the essential GSH precursor Cys 2 by culturing cells in Cys 2 -free culture medium or blocking de novo synthesis of GSH by using GCL inhibitor BSO or blocking Cys 2 uptake by pharmaceutical inhibition of system x c caused cell death