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Peptides were eluted over a 120-min gradient with buffer A as 100% water + 0.1% formic acid and buffer B as water + 0.1% formic acid and acetonitrile with the following steps: 05% B for 1 min, 525% for 68 min, 2540% for 20 min, 4060% for 15 min, 6095% for 1 min and 95% for 15 min

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