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A comprehensive panel of primary antibodies was used to detect various proteins: FOXO4, p53, Ser15-phospho-p53, p21 (cyclin-dependent kinase inhibitor 1A), p16 (cyclin-dependent kinase inhibitor 2A), 3-HSD, CYP11A1, CYP17A1 (cytochrome P450 family 17 subfamily A member 1), and several senescence-associated secretory phenotype (SASP) factors including IL-1, IL-1, IL-6, IL-10, TNF-, and TGF-
doi: 10.3389/fimmu.2025.1671548 Received 23 July 2025 Accepted 29 September 2025 Published 16 October 2025 Volume 16 - 2025 Edited by Yoshiro Kobayashi, Toho University, Japan Reviewed by Swarnima Pandey, University of Maryland, United States Jiaqian Luo, Fudan University, China Updates Copyright 2025 Zhao, Sun, Yang, Sun, Zhang, Sun, Yan and Wang
However, once a vial is punctured, its shelf life dramatically shortens
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