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The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea
In most cases: Injection site discomfort lasts a few hours to one day Mild headaches or nausea may last 2448 hours Skin sensitivity usually improves within a few days If symptoms persist for more than a few days, it is important to consult a healthcare professional
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